Title

Determination of heavy metals and aflatoxin M1 in traditional and industrial milk in Tehran city

Principal Investigator

Dr. Nabi Shariatifar

Abstract

چکیده لاتین Contamination of animal feed, climate with heavy metals is the main reason for the biological accumulation of these metals in biomass, especially plants and animal feed. This contamination can be transmitted to humans through foods such as milk and can cause side effects. Contamination of animal feed with aflatoxins also leads to contamination of livestock and due to the stability of aflatoxin M1 in the processing stages of milk such as pasteurization, sterilization, cheese and yogurt and other dairy products and due to the accumulation of mycotoxins in the body increases the risk of cancer. In order to measure heavy metals and aflatoxin M1 in industrial and traditional valves offered in Tehran. This descriptive-analytical study was performed in the years 1399-1400. To measure heavy metals, 83 milk samples were prepared, including 49 samples of industrial milk and 34 samples of traditional milk, of which 49 samples of industrial milk include 17 different brands of milk. To measure heavy metals, the samples were first reduced to ash, then acidic digestion and preparation for heavy metal measurement were injected into the ICP. To measure aflatoxin M1, 84 milk samples were selected, including 40 samples of industrial milk and 40 samples of traditional milk. All samples were then transferred to the laboratory and frozen. After defrosting, the milk fat was separated. The sample was prepared and after washing the immunophytic column, the amount of aflatoxin M1 was measured by HPLC-FLD method.
The amounts of arsenic, cadmium, cobalt, chromium, copper, iron, mercury, nickel, lead and zinc in the range of 1-0, 0.7-0, 0.43-41, 17.72-42-72, respectively. , 71 / 1544- 83/15, 21 / 3821- 80/109, 14 / 1- 0, 27 / 160- 46/8, 02 / 628-0, 77 / 3011- 71/320 in ppb. The average amount of aflatoxin M1 in all samples was 0.05125 ppb, which is lower than the Iranian National Standard limit (0.1 ppb) and the codex commission limit (0.5 ppb).
The concentration of heavy metals except lead which in traditional valves was higher than the national standard of Iran, the concentration of other metals in traditional and industrial valves was below the national standard of Iran. Also, the amount of aflatoxin M1 in the range was 0.005-0.174 ppb and the average of aflatoxin M1 was below the national standard of Iran.

Year

2020